Journal: Asian Pacific Journal of Cancer Prevention : APJCP
Article Title: Phosphorylated Akt Protein at Ser473 Enables HeLa Cells to Tolerate Nutrient-Deprived Conditions
doi: 10.22034/APJCP.2017.18.12.3255
Figure Lengend Snippet: Effect of Akt Inhibitor III Molecule on HeLa Cells Under Nutrient-Deprived Conditions is Concentration- and Time-Dependent. (A) Survival of HeLa cells treated with different concentrations (6.25, 12.5, and 25 μM) of Akt inhibitor III molecule in NDM for 24 hr expressed as percentage relative to that of cells cultured in NDM only (0 μM of Akt inhibitor III molecule). Data represent mean ± SD. (B) Survival of HeLa cells treated with 0 and 12.5 μM of Akt inhibitor III molecule in NDM at different time intervals expressed as percentage relative to cells treated with 0 μM of Akt inhibitor III molecule. Data represent mean ± SD. NDM, nutrient-deprived medium.
Article Snippet: The cells were seeded in triplicate at a density of 5×10 3 cells per well in 96-well plates and allowed to grow in fresh DMEM medium for 24 h. The cells were then washed with PBS (pH=7.4, Sigma-Aldrich, Inc) and the medium was changed to NDM containing different concentrations (0, 6.25, 12.5, or 25 µM) of Akt inhibitor III molecule (Calbiochem, CA, USA).
Techniques: Concentration Assay, Cell Culture